| dc.description.abstract | To screen for bacteriuria in women, a side centrifuge gram stain
was used.
4161 urine specimens were submitted in urine preservative tubes
for routine culture for slide centrifuges gram staining. Each urine
the sample was mixed thoroughly, then 0.2 ml was pipette-filled into a
slide centrifuge chamber and spun for 5 minutes at 2,000 rpm
The slides were heat-fixed, gram stained, and examined by
laboratory technicians who used a predefined pattern to scan 12
successive oil-immersion areas ds was of the
A positive urine screen was defined as the presence of the same
organism in six or more fields (E.coli was isolated in 75 percent of
the females under research). Urine samples were grown using a0.001
ml loop and comparisons were made.
According to a study published in 1956 by (Kass), gram stain
smears of uncentrifuged urine were positive in 80 percent of
specimens with bacteria counts of 100.000 or more CFU/ml.
use We developed the slides centrifuge to aid in the
the concentration of microorganisms for gram staining of body fluid
specimens and we tested its utility as a fast urine screening
the method in an adult female population.
The quantitative culture was used to compare the results.
Using the sequence illustrated in the image, 12))
successive fields on each slide were scanned
swiftly by oil immersion microscopy, starting in
the center of the slide centrifuge spot.))) | en_US |
