High performance liquid chromatography

High performance liquid chromatography (HPLC) is an important qualitative and quantitative technique, generally used for the estimation of pharmaceutical and biological samples. It is the most versatile, safest, dependable and fastest chromatographic technique for the quality control of drug components. Chromatography : physical method which separation of components takes place between two phasesa stationary phase and a mobile phase Stationary phase : The substance on which adsorption of the analyte (the substance to be separated during chromatography) takes place . Mobile phase : solvent which carries the analyte